ENFINIA DNA
The food and agriculture industry relies on metabolic engineering to develop transgenic plants capable of sustainably producing natural products that are challenging to chemically synthesize due to costs or structural complexity. Across cloning cycles, DNA is assembled into large multi-trait cassettes, sometimes >20kb in length, that produce the desired phenotypic behavior. ENFINIA DNA is delivered as long as 7kb and can be assembled directly into a larger construct with minimal or no cloning. This can reduce the time required to build long DNA constructs by 50%.
Elegen Standard Complexity | Elegen High Complexity | |
---|---|---|
DNA sequence length | 300 - 5.500bp | 300 - 7,000bp |
Overall GC content | 25 - 65% | 25 - 75% |
100bp GC content | 22 - 75% | 16 - 80% |
Local GC variation | up to 60% | up to 60% |
Repeats | up to 20bp | up to 100bp |
Homopolymers* | up to 7 bases for G/C and 8 bases for A/T | up to 10 bases for G/C and 20 bases for A/T |
* At this time, the QC methods we use for production do not provide high confidence in fidelity of A/T homopolymer sequences longer than 10bp. A/T homopolymer sequences up to 20bp will be accepted, but we cannot guarantee synthesis quality above 10bp. We are working to address this in the near future.